README for Figure S1.csv 
*** This file contains the raw data obtained on DNA and DNA-HMfB complexes using tethered particle motion represented in Figure S1 of 
Article: Specific DNA binding of archaeal histones HMfA and HMfB
Authors: Erkelens, Henneman, van der Valk, Kirolos and Dame
Journal:  
DOI:
Corresponding author: rtdame@chem.leidenuniv.nl;

Legend Figure S1:Calculated end-to-end distances for the unbound and bound population of 21 nM HMfB on 
Clone20 DNA. Histograms were fitted with a skewed normal distribution, resulting in end-to-end distances 
of 102.2 ± 10.4 nm and 78.64 ± 10.8 nm for unbound and bound DNA respectively. Insert: pairwise distribution 
plot of the differences between the two end-to-end distance peaks. Histogram was fitted with a Gaussian 
distribution resulting in a difference of 22.95 ± 9.3 nm. 

 *** The data were obtained using Tethered particle motion as described in the associated article. 


*** Data obtained for 21 nM HMfB is given in this table as plotted in figure S1.
Column A: Peak = Unbound or Bound state of the bead at 21 nM HMfB
Column B: Replicate = number of replicate
Column C: FoV = field of view (data with the same FoV were collected in one image) 
Column D: bead = beads in each FoV are represented by a unique number
Column E: 2.5% highest d (nm) = points with highest distance from center of the bead
Column F: end-to-end distance (nm) = distance calculated using d and triangular calculations
The values given in column A-D indicate which bead was selected and E and F are calculated as described in the associated article.